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Nucleic Acids Research, 1984, Vol. 12, No. 5 2317-2325
© 1984

MOLECULAR BIOLOGY

DNA sequence analysis of crown gall tumor T-DNA encoding the 0.7kb transcript

Joan C. McPherson

Department of Botany, University of British Columbia, Vancouver British Columbia V6T 2B1, Canada

Received January 3, 1984. Accepted February 14, 1984.

Crown gall tumor formation involves integration into the plant genome of DNA sequences (the T-region) of tumor-inducing (T1) plasmids present in Agrobacterium tumefaciens. The T-DNA of the tumor expresses several gene products. Little is know-n about the function or regulation of expression of the 0.7kb transcript, which represents a relatively abundant T-DNA transcript in octopine-type tumors. In this report, a detailed structural analysis of the gene encoding the 0.7kb transcript has been obtained by DNA sequence analysis of T-DNA isolated from A6S/2 tumor line. An indication of the structural characteristics of the protein product is obtained from the predicted amino acid sequence. The sequences flanking the open reading frame show characteristics with other eucaryotic genes. The corresponding DNA sequence of the inducing T1 plasmid (pT1A6) is identical with that of the DNA sequence from the tumor. Comparison of this gene sequence with the corresponding region of another T1 plasmid (pT1Ach5) shows several differences in the 5' flanking sequence, but the nucleotide sequence of the coding region and 3' flanking region are identical.


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