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Nucleic Acids Research, 1984, Vol. 12, No. 5 2381-2389
© 1984

CHEMISTRY

Critical amount of oligovalent Ion binding required for the B-Z transition of poly (dG-m5dC)

Holly Ho Chen1, Michael J. Behe2 and Donald C. Rau3

1Department of Chemistry, George Mason University 4400 University Drive, Fairfax, VA 22030 2Department of Chemistry, CUNY, Queens College Flushing, NY 11367 3Laboratory of Chemical Physics, NIADDKD, NIH Building 2, Room B1-03, Bethesda, MD 20205, USA

Received September 19, 1983. Accepted February 14, 1984.

By working at very low Na+ concentrations (1mM and less), the number of bound Mg(2+), cobalt hexamine(3+), and spermine(4+) necessary to induce the B-Z transition of poly (dG-m5 dC) has been directly measured. The results show that if as little as 1 cobalt hexarnine(3+) or spermine(4+) is bound per 40–50 nucleotides the transition will occur. A greater fraction of bound Mg(2+) is required, 1 bound Mg(2+)/10 nucleotides. The dependence of the transition midpoint concentrations of oligovalent ions on Na+ concentrations suggests that specific ion binding energies, not included in counterion condensation theory, are responsible for the transition.


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S. Thiyagarajan, S. S. Rajan, and N. Gautham
Cobalt hexammine induced tautomeric shift in Z-DNA: the structure of d(CGCGCA).d(TGCGCG) in two crystal forms
Nucleic Acids Res., November 8, 2004; 32(19): 5945 - 5953.
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