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Nucleic Acids Research, 1984, Vol. 12, No. 5 2581-2591
© 1984

CHEMISTRY

Computer programs in nucleic acid synthesis: synthetic strategy development using solid-phase chemical techniques with data storage, retrieval and analysis capabilities+

Stephen Lombardi*, Hollis Seidell, Stephen Pulford, Walter Dutton and Shirish Parekh

Oligonucleotides Section, Genex Corporation, Special Research Center 12300 Washington Avenue, Rockville, MD 20852, USA

Received July 11, 1983.

A computer program has been designed to aid development of synthetic strategies for oligonucleotides produced by solid-phase chemical techniques. The program reduces the time required to develop a strategy and a data file from hours to minutes. The program contains inventories, provides coat analyses, and generates and stores other associated data. The program searches an inventory of sequences for that sequence to avoid duplicate synthesis. If the sequence is not in the inventory the program devises a synthetic strategy, calculates the amounts of reagents and labor costs necessary to complete the synthetic oligonucleotide. The program also deducts the reagents from inventory files. Physical data is also calculated. A file is generated in a sequence inventory for storage of the data as well as other data that will be generated during the purification processes. All variable parameters can be easily edited. The programs were designed to provide a cross-referencing feature for data analysis and can use several parameters as a constant.

+This paper has been re-published from Nudeic Acids Research, 12, 437-446. The version which was initially published was incomplete as submitted. The missing page has now been supplied and the paper re-printed in its entirety.

*Present Address: Biotechnology Facilities, Agricultural Division, CIBA-Geigy Corporation, P.O. Box 12257, Research Triangle Park, North Carolina 27709


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