Nucleic Acids Research, 1984, Vol. 12, No. 6 2955-2968
© 1984
MOLECULAR BIOLOGY |
Structure and function of the nontranscribed spacer regions of yeast rDNA
1Inst. Molecular Biology, Academy of Sciences of the USSR Vavilovstr. 32, Moscow B-312, USSR 2Biochemisch Laboratorium, Vrije Universiteit, de Boelelaan 1083 1081 HV Amsterdam, The Netherlands 3Inst. Molecular Biology, Bulgarian Academy of Sciences 1113 Sofia, Bulgaria
*To whom requests for reprints should be sent
Received December 22, 1983. Revised February 24, 1984. Accepted February 24, 1984.
The sequences of the nontranscribed spacers (NTS) of cloned ribosomal DNA (rDNA) units from both Saccharomyces cerevisiae and Saccharomyces carlsbergensis were determined. The NTS sequences of both species were found to be 93% homologous. The major disparities comprise different frequencies of reiteration of short tracts of six to sixteen basepairs. Most of these reiterations are found within the 1100 basepairs long NTS between the 3'-ends of 26S and 5S rRNA (NTS1). The NTS between the starts of 5S rRNA and 37S pre-rRNA (NTS2) comprises about 1250 basepairs. The first 800 basepairs of NTS OTS2 Adjacent to the 5S rRNA gene) are virtually identical in both strains whereas a variable region is present at about 250 basepairs upstream of the RNA polymerase A transcription start. In contrast to the situation in Drosophila and Xenopus no reiterations of the putative RNA polymerase A promoter are present within the yeast NTS. The strands of the yeast NTS reveal a remarkable bias of G and C-residues. Yeast rDNA was previously shown to contain a sequence capable of autonomous replication (ARS) (Szostak, J.W. and Wu, R (1979) , Plasmid 2, 536–554). This ARS, which may correspond to a chromosomal origin of replication, was located on a fragment of 570 basepairs within NTS2.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Azvolinsky, S. Dunaway, J. Z. Torres, J. B. Bessler, and V. A. Zakian The S. cerevisiae Rrm3p DNA helicase moves with the replication fork and affects replication of all yeast chromosomes. Genes & Dev., November 15, 2006; 20(22): 3104 - 3116. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Grenetier, C. Bouchoux, and V. Goguel CTD kinase I is required for the integrity of the rDNA tandem array Nucleic Acids Res., October 18, 2006; 34(17): 4996 - 5006. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Johzuka, M. Terasawa, H. Ogawa, T. Ogawa, and T. Horiuchi Condensin Loaded onto the Replication Fork Barrier Site in the rRNA Gene Repeats during S Phase in a FOB1-Dependent Fashion To Prevent Contraction of a Long Repetitive Array in Saccharomyces cerevisiae. Mol. Cell. Biol., March 1, 2006; 26(6): 2226 - 2236. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. R. D. Ganley, K. Hayashi, T. Horiuchi, and T. Kobayashi Identifying gene-independent noncoding functional elements in the yeast ribosomal DNA by phylogenetic footprinting PNAS, August 16, 2005; 102(33): 11787 - 11792. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Takeuchi, T. Horiuchi, and T. Kobayashi Transcription-dependent recombination and the role of fork collision in yeast rDNA Genes & Dev., June 15, 2003; 17(12): 1497 - 1506. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Weitao, M. Budd, L. L. M. Hoopes, and J. L. Campbell Dna2 Helicase/Nuclease Causes Replicative Fork Stalling and Double-strand Breaks in the Ribosomal DNA of Saccharomyces cerevisiae J. Biol. Chem., June 13, 2003; 278(25): 22513 - 22522. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. S. Ivessa and V. A. Zakian To fire or not to fire: origin activation in Saccharomyces cerevisiae ribosomal DNA Genes & Dev., October 1, 2002; 16(19): 2459 - 2464. [Full Text] [PDF]
|
|
|
|
|
|
M. Gruber, R. E. Wellinger, and J. M. Sogo Architecture of the Replication Fork Stalled at the 3' End of Yeast Ribosomal Genes Mol. Cell. Biol., August 1, 2000; 20(15): 5777 - 5787. [Abstract] [Full Text]
|
|
|
|
|
|
K. T. D. Burkard and J. S. Butler A Nuclear 3'-5' Exonuclease Involved in mRNA Degradation Interacts with Poly(A) Polymerase and the hnRNA Protein Npl3p Mol. Cell. Biol., January 15, 2000; 20(2): 604 - 616. [Abstract] [Full Text]
|
|
|
|
|
|
M. Banditt, T. Koller, and J. M. Sogo Transcriptional Activity and Chromatin Structure of Enhancer-Deleted rRNA Genes in Saccharomyces cerevisiae Mol. Cell. Biol., July 1, 1999; 19(7): 4953 - 4960. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. S. Smith, E. Caputo, and J. D. Boeke A Genetic Screen for Ribosomal DNA Silencing Defects Identifies Multiple DNA Replication and Chromatin-Modulating Factors Mol. Cell. Biol., April 1, 1999; 19(4): 3184 - 3197. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kobayashi, D. J. Heck, M. Nomura, and T. Horiuchi Expansion and contraction of ribosomal DNA repeats in Saccharomyces cerevisiae: requirement of replication fork blocking (Fob1) protein and the role of RNA polymerase I Genes & Dev., December 15, 1998; 12(24): 3821 - 3830. [Abstract] [Full Text]
|
|
|
|
 |
|
 J. S. Smith, C. B. Brachmann, L. Pillus, and J. D. Boeke Distribution of a Limited Sir2 Protein Pool Regulates the Strength of Yeast rDNA Silencing and Is Modulated by Sir4p Genetics, July 1, 1998; 149(3): 1205 - 1219. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J S Smith and J D Boeke An unusual form of transcriptional silencing in yeast ribosomal DNA. Genes & Dev., January 15, 1997; 11(2): 241 - 254. [Abstract] [PDF]
|
|
|
|
|
|
E. B. Mougey, L. K. Pape, and B. Sollner-Webb Virtually the Entire Xenopus laevis rDNA Multikilobase Intergenic Spacer Serves to Stimulate Polymerase I Transcription J. Biol. Chem., October 25, 1996; 271(43): 27138 - 27145. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M E Schmitt and D A Clayton Yeast site-specific ribonucleoprotein endoribonuclease MRP contains an RNA component homologous to mammalian RNase MRP RNA and essential for cell viability. Genes & Dev., October 1, 1992; 6(10): 1975 - 1985. [Abstract] [PDF]
|
|
|
|
 |
|
 V. Parikh, M. Morgan, R Scott, L. Clements, and R. Butow The mitochondrial genotype can influence nuclear gene expression in yeast Science, January 30, 1987; 235(4788): 576 - 580. [Abstract] [PDF]
|
|