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Nucleic Acids Research, 1984, Vol. 12, No. 7 3089-3096
© 1984

CHEMISTRY

Interaction and conformational changes of chromatin with divalent ions

Nina Borochov, Juan Ausio and Henryk Eisenberg

Department of Polymer Research, The Weizmann Institute of Science Rehovot 76100, Israel

Received January 17, 1984. Revised March 15, 1984. Accepted March 15, 1984.

We have investigated the interaction of divalent ions with chromatin towards a closer understanding of the role of metal ions in the cell nucleus.

The first row transition metal ion chlorides MnCl2, CoCl2, NiCl2 and CuCl2 lead to precipitation of chicken erythrocyte chromatin at a significantly lower concentration than the alkali earth metal chlorides MgCl2, CaCl2 and BaCl2. A similar distinction can be made for the compaction of chromatin to the "30 nm" solenoid higher order structure which occurs at lower MeCl2 concentration in the first group but at the same MeCl2 concentration within each group. In other experiments in which mixed solutions of NaCl and of MgCl2 were examined, it is shown that increasing NaCl concentration leads to increasing solubility in the presence of MgCl2. Best compaction of chromatin was obtained at 40 mM NaCl and 0.8 mM MgCl2 at a value A260 approximately 0.8. Similar experiments were undertaken with mixtures of NaCl and MnCl2.


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