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Nucleic Acids Research, 1984, Vol. 12, No. 9 3959-3971
© 1984

MOLECULAR BIOLOGY

The nucleotide sequence of the chicken thymidine kinase gene and the relationship of its predicted polypeptide to that of the vaccinia virus thymidine kinase

T.Jesse Kwoh and Jeffrey A. Engler1

Department of Veterinary Microbiology, University of Saskatchewan Saskatoon, Saskatchewan S7N OWO, Canada 1Department of Biochemistry, University of Alabama in Birmingham, University Station Birmingham, AL 35294, USA

Received February 20, 1984. Accepted April 11, 1984.

The entire DNA nucleotide sequence of a 3.0 kilobase pair Hind I I I fragment containing the chicken cytoplasmic thymldine kinase gene was determined. Ollgonucleotide linker insertion mutations distributed throughout this gene and having known effects upon gene activity (Kwoh, T.J., Zipser, D., and Wigler, M. 1983. J. Mol. Appl. Genet. 2, 191–200), were used to access regions of the Hind I I I fragment for sequencing reactions. The complete nucleotide sequence, together with the positions of the linker insertion mutations within the sequence, allows us to propose a structure for the chicken thymldine kinase gene. The protein coding sequence of the gene is divided into seven small segments (each less than 160 base pairs) by six small introns (each less than 230 base pairs). The proposed 244 amino acid polypeptide encoded by this gene bears strong homology to the vaccinia virus thymldine kinase. No homology with the thymldine kinases of the herpes simplex viruses was found.


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