Skip Navigation

This Article
Right arrow Print PDF (3144K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (20)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Dorfman, D. M.
Right arrow Articles by Domelson, J. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Dorfman, D. M.
Right arrow Articles by Domelson, J. E.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 10 3533-3549
© 1985

Articles

The 5.8S ribosomal RNA gene of Trypannsoma brucei: structural and transciiptional studies

David M. Dorfman+,*, Michael J. Lenardo+, Lekkala V. Reddy+, Lex H. T. Van Der Ploeg§ and John E. Domelson+,*

+Department of Biochemistry, University of Iowa Iowa City, IA 52242 *PhD Program in Genetics, University of Iowa Iowa City, IA 52242 §Department of Human Genetics and Development, College of Physicians and Surgeons, Columbia University 701 West 168th Street, New York, NY 10032, USA

Received January 30, 1985. Revised April 26, 1985. Accepted April 26, 1985.

To further investigate the process of discontinuous transcription in trypanosomes, the 5.8S rRNA gene, present in the trypanosome genome as part of the multicopy rRNA gene cluster, has been cloned, sequenced, chromosomally mapped, and used in transcriptional studies. The gene's sequence confirms its identity and indicates that it is less conserved evolutionarily than the trypanosome 5S rRNA gene previously described by our laboratory (6). Examination of the chromosomal locations of the gene by pulsed-field gradient gel electrophoresis shows that the 5.8S rRNA genes occur on at least four differently-sized chromosomes in T. b. rhodesiense and at least three differently-sized chromosomes in T. b. brucei The 5.8S transcript was analyzed by a run-off transcription assay using isolated nuclei. These studies strongly suggest that the 5.8S rRNA gene is transcribed as part of a larger precursor rRNA independently of the spliced leader RNA and by a different RNA polymerase than either the spliced leader or 5S rRNA transcripts. Transcription of the trypanosome 5.8S rRNA is insensitive to very high levels of alpha-amanitin, a feature of the 5.8S rRNA in higher eukaryotes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
H. Zhang, Y. Hou, L. Miranda, D. A. Campbell, N. R. Sturm, T. Gaasterland, and S. Lin
Spliced leader RNA trans-splicing in dinoflagellates
PNAS, March 13, 2007; 104(11): 4618 - 4623.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. J. DeStefano and J. J. DeStefano
Interaction of Human Immunodeficiency Virus Nucleocapsid Protein with a Structure Mimicking a Replication Intermediate. EFFECTS ON STABILITY, REVERSE TRANSCRIPTASE BINDING, AND STRAND TRANSFER
J. Biol. Chem., July 5, 1996; 271(27): 16350 - 16356.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
J. Walder, P. Eder, D. Engman, S. Brentano, R. Walder, D. Knutzon, D. Dorfman, and J. Donelson
The 35-nucleotide spliced leader sequence is common to all trypanosome messenger RNA's
Science, August 1, 1986; 233(4763): 569 - 571.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.