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Nucleic Acids Research, 1985, Vol. 13, No. 11 3969-3978
© 1985

Articles

Pattern of undermethylation of the major satellite DNA of mouse sperm

Sheldon I. Feinstein1,4, Vincent R. Racaniello2,4, Melanie Ehrlich5, Charles W. Gehrke6, Dorothy A. Miller1,4 and Orlando J. Miller1,3,4

1Department of Human Genetics and Development, Columbia University New York, NY 10032 2Department of Microbiology, Columbia University New York, NY 10032 3Department of Obstetrics and Gynecology, Columbia University New York, NY 10032 4The Cancer Center, Columbia University New York, NY 10032 5Department of Biochemistry, Tulane University New Orleans, LA 70112 6Department of Biochemistry, Experiment Station Chemical Laboratories, University of Missouri Columbia, MO 65201, USA

Received March 8, 1985. Accepted April 30, 1985.

Enzymatic hydrolysis and base analysis by high performance liquid chromatography showed that mouse satellite DNA had 30–50% less 5-methylcytosine in sperm than in somatic tissue (1.59 mols Z vs 2.40-3.11 mols Z). Maxam-Gilbert sequencing and analysis of the intensity of the cytosine bands indicated that the level of methylation of the eight CpGs of the consensus sequence in sperm satellite DNA ranged from 0 to about 50%, considerably lower than the levels reported in somatic tissues. The MnlI site containing one of these CpGs was cut much more extensively in satellite DNA from sperm than from liver, confirming the undertmethylation of this site in sperm DNA.


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