Skip Navigation

This Article
Right arrow Print PDF (4111K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Plumb, M.A.
Right arrow Articles by Goodwin, G.H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Plumb, M.A.
Right arrow Articles by Goodwin, G.H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 11 4047-4065
© 1985

Articles

Multiple sequence-specific DNA binding activities are eluted from chicken nuclei at low ionic strengths

M.A. Plumb, R.H. Nicolas, C.A. Wright and G.H. Goodwin

Chester Beatty Laboratories, Institute of Cancer Research Royal Cancer Hospital, Fulham Road, London SW3 6JB, UK

Received February 12, 1985. Accepted May 2, 1985.

DNA sequence-specific binding proteins eluted from chicken erythrocyte and thymus nuclei, and fractionated as described by Emerson and Felsenfeld (19), have been investigated by filter binding and footprint analyses. The erythrocyte nuclear protein fraction specifically binds to at least two sites within the 5' flanking chromatin hypersensitive site of the chicken ßA-glob1n gene, and to a site 5' to the human ß-glob1n gene. The major chicken ßA globin gene binding site ((G)gCGGGTGG) and the human ß-glob1n gene binding site ((TA)6(T)8C(T)4) occur at or near sequences which are hypersensitive to SI nyclease cleavage in supercoiled plasmids. Downstream, the second chicken ßA-globin gene binding site includes the ß-globin gene CACCC consensus sequence. Filter binding studies also show other sequence specific binding activities to human N-ras and human (but not chicken) c-myc gene sequences


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
K P Foley and J D Engel
Individual stage selector element mutations lead to reciprocal changes in beta- vs. epsilon-globin gene transcription: genetic confirmation of promoter competition during globin gene switching.
Genes & Dev., May 1, 1992; 6(5): 730 - 744.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.