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Nucleic Acids Research, 1985, Vol. 13, No. 13 4983-4989
© 1985

Articles

Evidence for Z-form RNA by vacuum UV circular dichroism

Jeannine H. Riazance, Walter A. Baase*, W. Curtis Johnson, Jr., Kathleen Hall, Phillip Cruz and Ignacio Tinoco, Jr.

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA Chemistry Department and Laboratory of Chemical Biodynamics, University of California Berkeley, CA 94720, USA

Received March 11, 1985. Revised June 3, 1985. Accepted June 3, 1985.

Circular dichroism (CD) spectra in the vacuum UV region for different conformations of poly d(G-C).poly d(G-C) and poly r(G-C)·poly r(G-C) are very characteristic. The CD of the RNA in the A-form (6 N NaClO4 and 22°C) is very similar to that of the DNA in 80% alcohol where it is believed to be in the A-form. With the exception of the longest wavelength transition, the CD of the RNA in 6 N NaClO4 at 46°C is similar.ar to the CD of the DNA under conditions where it is believed to be in the Z-form (2 M NaClO4 This substantiates that poly r(G-C)·poly r(G-C) assumes a left-handed Z-conformation in 6 M NaClO4 above 35°C. CD spectra for the left-handed Z-forms of both the RNA and DNA are characterized by an intense negative peak at 190–195 nm, a crossover at about 184 nm, and an intense positive peak below 180 nm. The right-handed A- and B-forms of RNA and DNA all have an intense positive peak in their CD spectra near 186 nm. The large difference in CD in the range 185–195 rim for right- and left-handed conformations of nucleic acids can be used to identify the sense of helix winding.

*Present address: Institute of Molecular Biology, University of Oregon, Eugene, OR 97403, USA


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