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Nucleic Acids Research, 1985, Vol. 13, No. 16 5761-5770
© 1985

Articles

Regional mapping of two human immunoglobulin V{lambda} genes and analysis of theV{lambda} locus in chronic myeloid leukaemia

M.L.M. Anderson, M.H. Goyns, A.M.H. Geurts van Kessel1 and B.D. Young2

Beatson Institute, Garscube Estate Switchback Road, Bearsden, Glasgow, UK, 1Department of Cell Biology and Genetics, Erasmus University P.O. Box 1738-3000 DR Rotterdam, The Netherlands 2Imperial Cancer Research Fund Medical Oncology Unit, St. Bartholomew's Hospital Little Britain, London EC1, UK

Revised August 2, 1985. Accepted August 2, 1985.

The human immunoglobulin V{lambda} locus has been studied in relation to chromosomal translocations involving chromosome 22. DNA probes for two V{lambda} genes which belong to different subgroups and do not cross hybridize, were used to show that both V{lambda} genes are located on the Philadelphia chromosome in chronic myeloid leukaemia (CML). Both genes map in band 22q11 to a region that is bounded on the distal side by the breakpoints for CML 9:22 translocations and on the proximal side by the breakpoint for an X:22 translocation. We have found no evidence for rearrangements or amplification of either V{lambda} gene in CML, in either the chronic or acute phases of the disease. In K562 cells which are derived from the pleural effusion of a patient with Ph1–positive CML, there appears to be no rearrangement of the V{lambda}genes, but they are both amplified about four times. We have estimated that the minimum size for the amplification unit in K562 cells is 186 kb.


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