Skip Navigation

This Article
Right arrow Print PDF (1596K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (26)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lapeyre, B.
Right arrow Articles by Amalric, F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lapeyre, B.
Right arrow Articles by Amalric, F.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 16 5805-5816
© 1985

Articles

Cloning of cDNA encoding a 100 kDa nudeolar protein (nucleoline) of Chinese hamster ovary cells

Bruno Lapeyre, Michèle Caizergues-Ferrer, Gérard Bouche and François Amalric*

Centre de Recherche de Biochimie et de Génétique Cellulaires du CNRS 118 route de Narbonne,31062 Toulouse Cedex, France

*To whom correspondence should be addressed

Received April 9, 1985. Revised June 20, 1985. Accepted July 30, 1985.

Nucleoline (100 kDa) is the major nucleolar protein in exponentially growing cells that behaves like a nucleolar organizer protein and plays a key role in rDNA transcription and prerRNA processing. We reported the isolation of 5 cDNA clones by probing a cDNA library, constructed in the expression vector {lambda} gt11, with a polyclonal serum raised against nudeoline. A new immunoassay, using hybrid proteins (ßgal-cDNA encoded protein)was developped to establish that the isolated cDNAs encoded parts of nucleoline. A further confirmation resulted from the sequence comparison between the cDNA encoded peptide and a 42 aa peptide isolated from rat nudeoline (1). The 5 cDNAs overlapped extensively and covered more than 90 % of a full length cDNA. By probing a Northern blot with the 100kDa cDNA, a 2650 nucleotide polyA+ RNA was detected that contained just enough information to code for nucleoline.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
T. Uinuk-ool, W. E. Mayer, A. Sato, R. Dongak, M. D. Cooper, and J. Klein
Lamprey lymphocyte-like cells express homologs of genes involved in immunologically relevant activities of mammalian lymphocytes
PNAS, October 29, 2002; 99(22): 14356 - 14361.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
H Ginisty, H Sicard, B Roger, and P Bouvet
Structure and functions of nucleolin
J. Cell Sci., January 3, 1999; 112(6): 761 - 772.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
P. Bouvet, J.-J. Diaz, K. Kindbeiter, J.-J. Madjar, and F. Amalric
Nucleolin Interacts with Several Ribosomal Proteins through Its RGG Domain
J. Biol. Chem., July 24, 1998; 273(30): 19025 - 19029.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Tang, J. D. Gary, S. Clarke, and H. R. Herschman
PRMT 3, a Type I Protein Arginine N-Methyltransferase That Differs from PRMT1 in Its Oligomerization, Subcellular Localization, Substrate Specificity, and Regulation
J. Biol. Chem., July 3, 1998; 273(27): 16935 - 16945.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M Caizergues-Ferrer, P Mariottini, C Curie, B Lapeyre, N Gas, F Amalric, and F Amaldi
Nucleolin from Xenopus laevis: cDNA cloning and expression during development.
Genes & Dev., March 1, 1989; 3(3): 324 - 333.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
J. Rho, S. Choi, Y. R. Seong, W.-K. Cho, S. H. Kim, and D.-S. Im
PRMT5, Which Forms Distinct Homo-oligomers, Is a Member of the Protein-arginine Methyltransferase Family
J. Biol. Chem., March 30, 2001; 276(14): 11393 - 11401.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.