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Nucleic Acids Research, 1985, Vol. 13, No. 16 5937-5948
© 1985

Articles

Cloned DNA sequences that determine mRNA stability of bacteriophage FEX174 in vivo are functional

Marie N. Hayashi and Masaki Hayashi*

Department of Biology, B-022 University of California San Diego, La Jolla, CA 92093, USA

*To whom correspondence should be addressed

Received April 30, 1985. Revised July 22, 1985. Accepted July 22, 1985.

The stability of two species of øx174 polycistronio mRNA in vivo can be altered by mutating sequences existing immediately upstream of a termination site. The wild type phage contains an mRNA stabilixing sequence ((+) sequence), while the same sequence mutated by insertion ((–) sequence) reduces the stability of the mRNAs. These two sequences were cloned at the 3' ends of gene D or gene B of øX174 in a pBR322 derivative plasmid. The cloned sequences were functional. The (+) sequence stabilized gene B or gene D mRNA; half-lives of these mRNAs were 7 to 8 min. When the (+) sequence is eliminated ((o) sequence) or replaced with the (–) sequence, the half-lives of the mRNA were reduced to about 1 to 2 min. The stabilisation of mRNAs caused an increased production of these proteins.


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