Nucleic Acids Research, 1985, Vol. 13, No. 16 5965-5976
© 1985
Articles |
Analysis of the 5' regulatory region of the gene for 
-aminolevulinic acid synthetase of Rhizobium meliloti
USDA-ARS, Department of Plant Pathology, University of Wisconsin Madison, WI 53706 USA 1Department of Genetics, University of Leicester Leicester, UK 2Department of Biology B-022, University of California La Jolla, CA 92093, USA
Received April 1, 1985. Revised July 23, 1985. Accepted July 23, 1985.
Transcriptional regulation of the 
-aminolevulinic acid synthetase gene of Rhizobium meliloti was investigated under conditions of nornal vegetative growth and during symbiosis with the legume host alfalfa. S1 nuclease mapping and DHA sequence analysis indicated that transcription originates from two sites separated by 238 base pairs. A deletion analysis of the putative promoter regions P1 and P2, corresponding to the proximal and diatal RNA start sites, was carried out with Bal-31 nuclease. Promoter function was monitored as ß-galactosidase activity after fusing the deletions to lac Z and introducing them into Rhizobium on a broad host range plasmid. The data obtained suggest that both regions function equivalently as promoters. The DNA sequences of P1 and P2 show considerable homology in the region between –35 and the start of transcription. Both contain a –35 region that is analogous to the consensus E. coli promoter sequence, while the –10 region is dissimilar. No resemblance was found between either P1 or P2 and the promoter regions of genes under general nitrogen control.
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