Nucleic Acids Research, 1985, Vol. 13, No. 17 6049-6057
© 1985
Articles |
Cloning and the nucleotide sequence of rat glutathione S-transferase P cDNA
Department of Biochemistry, The University of Tokyo Faculty of Medicine Hongo, Bunkyo-ku, Tokyo, 113 *Department of Pathology, Cancer Institute Kamiikebukuro, Toshima-ku, Tokyo 170, Japan
Received July 8, 1985. Revised August 13, 1985. Accepted August 13, 1985.
A cDNA library prepared from poly(A)+ RNA of 2-acetylaminofluorene (AAF) induced rat hepatocellular carcinoma was screened by synthetic DNA probes deduced from a partial amino acid sequence of glutathione S-transferase P subunit that had been isolated from the tumor by two-dimensional gel electrophoresis. One of the four clones analyzed contained an mRNA region encoding the total amino acid sequence of this enzyme subunit and the complete 3'-noncoding region. The nucleotide sequence indicates that this enzyme subunit has 209 amino acids (calculated Mr=23,307) distinct from other glutathione S-transferase subunits such as Ya and Yc. Comparison of the amino acid sequences between these proteins indicates that glutathione S-transferase P subunit gene has been evolved from the ancestral gene at an earlier stage than the separation of Ya and Yc and that there are at least three domains having a considerable homology with each other in these enzymes. The very large increase of this mRNA in chemically induced hepatocellular carcinoma suggests a characteristic derepression of this gene during hepatocarcinogenesis.
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