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Nucleic Acids Research, 1985, Vol. 13, No. 17 6265-6272
© 1985

Articles

Suppression of a nonsense mutation in mammalian cells in vivo by the aminoglycoside anthiotics G–418 and paromomycin

Julian F. Burke1 and Anne E. Mogg1,2

1 School of Biological Sciences, and MRC Cell Mutation Unit, University of Sussex Falmer, Brighton BN1 9QG, UK 2 MRC Cell Mutation Unit, University of Sussex Falmer, Brighton BN1 9QG, UK

Received July 5, 1985. Revised August 15, 1985. Accepted August 15, 1985.

Aminoglycoside antibiotics in Escherichia coli and yeast can cause ribosomes to read through stop codons during translation. This can result in the phenotypic suppression of nonsense nutations. We show here for the first time that the aminoglycosides G–418 and paronomycin have similar effects in monkey (COS–7) cells in vivo. Suppression of an amber mutation (TAG) by aninoglycosides can restore the activity of a mutant gene transfected into COS–7 cells to almost 20% of wild type levels.


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