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Nucleic Acids Research, 1985, Vol. 13, No. 18 6545-6558
© 1985

Articles

Developmental modulation of DNA methylatlon in the fungus Phycomyces blakesleeanus

Francisco Antequera*, Mercedes Tamame, Julio R. Villanueva and Tomás Santos

Departamento de Microbiología, Facultad de Biología, Universidad de Salamanca 37071 Salamanca, Spain

*To whom correspondence should be addressed

Received July 1, 1985. Revised September 6, 1985. Accepted September 6, 1985.

DNA methylation is a rather sparse event among fungi. Phycomyces blakesleeanus seems to be one of the few exceptions in this context. 5-Methylcytosine represents 2.9% of the total cytosine in spore DNA and is located in approximately the same amount at any of the four CA, CT, CC or CG dinucleotides. A progressive and gradual drop in total 5-methylcytosine parallels the development of the fungus. This demethylation is non random but sequence specific and is not accounted for equally by the four different methylated dinucleotides, CG being much less affected (20% demethylated) than CA, CT and CC (more than 90% demethylated at the same time). "De novo" methylation to restore the initial pattern probably takes place during spore maturation. By using specific hybridization probes we have been able to show that the rRNA genes are not significantly methylated at any stage of development, regardless of their transcription status.


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