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Nucleic Acids Research, 1985, Vol. 13, No. 19 6797-6816
© 1985

Articles

Poliovirus genome RNA hybridizes specifically to higher eukaryotic rRNAs

Marcella A. McClure*,+ and Jacques Perrault+

Department of Microbiology and Immunology, Washington University School of Medicine St. Louis, MO 63110 and Molecular Biology Institute and Biology Department, San Diego State University San Diego, CA 92182, USA

*To whom reprint requests should be addressed

Received August 28, 1985. Accepted September 12, 1985.

The RNA genome of poliovirus hybridizes to 28S and 18S rRNAs of higher eukaryotes under stringent conditions. The hybridization detected by Northern blot analyses is specific since little or no signal was detected for yeast or prokaryotic rRNAs or other major cellular RNAs. Southern blot analysis of DNA clones of mouse rRNA genes leads us to conclude that several regions of 28S rRNA, and at least one region in 18S rRNA, are involved in the hybridization to polio RNA, and that G/C regions are not responsible for this phenomenon. We have precisely mapped one of these hybridizing regions in both molecules. Computer analysis confirms that extensive intermolecular base-pairing (81 out of 104 contiguous bases in the rRNA strand) could be responsible for this one particular site of interaction (polio genome, bases 5075–5250; 28S rRNA, bases 1097–1200). We discuss the possible functional and/or evolutionary significance of this novel type of interaction.

+Present address: Biology Department, San Diego State University, San Diego, CA 92182.


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