Skip Navigation

This Article
Right arrow Print PDF (653K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kita, K.
Right arrow Articles by Saito, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kita, K.
Right arrow Articles by Saito, H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 19 7015-7024
© 1985

Articles

Interaction of the restriction endonuclease ScaI with its substrates

Keiko Kita, Nobutsugu Hiraoka, Fusao Kimizuka, Akira Obayashi, Hiroyuki Kojima*, Hideo Takahashi* and Hiuga Saito*

Central Research Laboratories, Takara Shuzo Co., Ltd. Seta 3-4-1, Otsu-shi, Shiga 520-21 *Institute of Applied Microbiology, University of Tokyo Bunkyo-ku, Tokyo 113, Japan

Received June 12, 1985. Revised September 9, 1985. Accepted September 16, 1985.

The kinetic conatants of the site-specific endonuclease, ScaI, for various substrates were determined. We estimated Vmax and Km for octa-, deca-, dodeca-, and hexadecanucleotides and for plasmid pBR322 DNA. Vmax for these substrates were close, but Km were quite different (in decreasing order, octa- > deca-, dodeca-, hexadeca- > pBR322). The results were discussed with respect to the tertiary structure of substrate.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.