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Nucleic Acids Research, 1985, Vol. 13, No. 2 369-379
© 1985

Articles

The gene for an exported antigen of the malaria parasite Plasmodium falciparum cloned and expressed in Escherichia coli

Ian A. Hope*, Martin Mackay, John E. Hyde**, Mike Goman and John Scaife+

Department of Molecular Biology, University of Edinburgh Kings Buildings, Mayfield Road, Edinburgh EH9 3JR, UK *Department or Biological Chemistry, Harvard Medical School Shattuck St, Boston, Mass 02115, USA **Department of Biochemistry and Applied Molecular Biology UMIST PO Box 88, Manchester M60 1QD, England

+To whom correspondence should be addressed

Received November 19, 1984. Revised December 19, 1984. Accepted December 19, 1984.

An exported protein of the erythrocytic stages of the malaria parasite, Plasmodium falciparum has epitope(s) in common with the surface of the sporozoite stage (1). Two cDNA clones encoding this protein, Ag5.1, have now been isolated and expressed in Escherichia coli. The coding sequence contains a region with strong homology to that of the circumsporozoite protein of P.falciparum. Other features of the sequence can be explained in terms of the observed behaviour of the protein in the parasite life cycle. The Ag5. 1 can now be synthesised in bacteria in sufficient amounts to analyse the immune response to this protein.


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