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Nucleic Acids Research, 1985, Vol. 13, No. 20 7223-7236
© 1985

Articles

Molecular cloning and nucleotide sequence of a developmentally regulated gene from the cyanobacterium Calothrix PCC 7601: a gas vesicle protein gene

Nicole Tandeau de Marsac*, Didier Mazel, Donald A. Bryant+ and Jean Houmard

Unité de Physiologie Microbienne, Département de Biochimie et Génétique Moléculaire, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France +Department of Molecular and Cell Biology, S-101 Frear Building, The Pennsylvania State Univesity University Park, PA 16802, USA

*To whom correspondence should be addressed

Received August 16, 1985. Accepted September 30, 1985.

Since the gas vesicle protein (GVP) is highly conserved among the different gas-vacuolate prokaryotes, a 29-mer oligonucleotide corresponding to a portion of the Anabaena flos-aquae GVP gene was synthesized and used to isolate the GVP structural gene from Calothrix PCC 7601 (= Fremyella diplosiphon). Gas vacuole production in this filamentous cyanobacterium is restricted to hormogonia which occur at a specific stage during the developmental cell cycle. The GVP gene (gvpA) was localized on a 709 bp HindIII-HincII fragment. Nucleotide sequence analysis revealed a 213 bp open reading frame whose deduced amino-acid sequence shows a very high homology with that of the Anabaena flos-aquae GVP. Assuming that the first methionine residue is proteolytically processed, the molecular mass of the Calothrix GVP is 7375 daltons. Sequences resembling the Escherichia coli consensus promoter were found upstream from the gvpA gene. The initiator codon of the gvpA gene is preceded by a polypurine sequence assumed to be the ribosome binding site. Southern hybridizations with a probe specific for the gvpA gene indicated that this gene is not plasmid-borne, and that another homologous gene is present in the Calothrix genome.


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