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Nucleic Acids Research, 1985, Vol. 13, No. 20 7289-7297
© 1985

Articles

Attenuation and processing of RNA from the rpsO-pnp transcription unit of Escherichia coli

R. Takata, T. Mukai* and K. Hori*

Department of Biology, Saga Medical School Nabeshima, Saga 840-01, Japan *Department of Biochemistry, Saga Medical School Nabeshima, Saga 840-01, Japan

Received July 29, 1985. Accepted September 18, 1985.

Ribosomal protein S15 and polynucleotide phosphorylase of E.coli are encoded by two adjacent genes, rps0 and pnp, respectively. Analysis of in vivo transcripts from these two genes shows that they are within the same operon (S15 operon). By correlating the 5' and 3' ends of their in vivo transcripts with the DNA sequence, we have identified several features of the operon structure. These features include a promotor upstream from rps0 an attenuator downstream from rps0 and an RNA processing site between these two genes.


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