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Nucleic Acids Research, 1985, Vol. 13, No. 22 8019-8033
© 1985

Articles

Genomic cloning and characterization of a ricin gene from Ricinus Communis

Kevin C. Halling, Amy C. Halling, Elizabeth E. Murray, Beth F. Ladin, L.L. Houston* and Robert F. Weaver

University of Kansas, Department of Biochemistry Lawrence, KS 66044, USA

Received August 26, 1985. Accepted October 15, 1985.

A genomic clone that specifies a single poypeptide precursor for ricin, a toxic lectin of Ricinus communis (castor bean), was isolated, sequenced and S1 mapped. The gene encodes a 64 kDa precursor which contains, in the following order: a 24 or 35 amino acid signal peptide, the A chain, a 12 amino acid linker peptide, and the B chain. The 5'-end of the ricin mRNA maps approximately 35 bases upstream from the first methionine codon. Two putative TATA boxes and a possible CAAT box lie in the 5'-flanking region. Two possible polyadenylation signals are found in the 3' flanking region. No introns were found, which is typical of other lectin genes that have been sequenced. Southern blot analysis suggests that the castor bean genome contains approximately six ricin-like genes.

*Present address: Cetus Corporation, Emeryville, CA, USA


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