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Nucleic Acids Research, 1985, Vol. 13, No. 22 8053-8063
© 1985

Articles

Selection of Sequence elements that substitute for the standard AATAAA motif which signals 3' processing and polyadenylation of late simian virus 40 mRNAs

Candace Swimmer1 and Thomas Shenk2,*

Department of Microbiology, Health Science Center, State University of New York Stony Brook, NY 11794, USA

*To whom correspondence should be addressed

Received August 19, 1985. Revised October 14, 1985. Accepted October 15, 1985.

A method is described which allows selection of sequences which can substitute for the normal AATAAA hexanucleotide involved in polyadenylation of SV40 late mRNAs. Plaques were generated from viral DNA lacking the motif, forcing acquisition of substitute sequences. Four variants wre characterized. All displayed wild-type growth kinetics and produced normal levels of late mRNAs and proteins. Two variants had reacquired AATAAA elements and one acquired an ATTAAA sequence. The last variant carried an ATTTTTTAAA segment, suggesting this novel sequence, or some portion of it, can also signal poly A addition.

1Present address: The Biological Laboratories, Harvard University, Cambridge, MA 02138, USA

2Present address: Department of Molecular Biology, Princeton University, Princeton, NJ 08544, USA


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