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Nucleic Acids Research, 1985, Vol. 13, No. 22 8105-8118
© 1985

Articles

Methylation of the alphafetoprotein gene in cell populations isolated from rat livers during carcinogenesis

Christos J. Petropoulos*, Paul Yaswen+, Marilyn Panzica and Nelson Fausto§

Department of Pathology and Laboratory Medicine, Division of Biology and Medicine, Brown University Providence, RI 02912, USA

§To whom correspondence should be addressed

Received June 20, 1985. Revised September 18, 1985. Accepted October 23, 1985.

We examined the methylation pattern and organization of the AFP gene in whole livers and in isolated cell populations purified from livers of rats fed a carcinogenic diet which interferes with DNA methylation. Using restriction endonuclease digestion, we find no differences in methylation pattern and overall organization of the AFP gene in oval cells (AFP-producers) and hepatocytes (non-producers) isolated at the early stages of carcinogenesis. Our studies indicate that in cell populations which produce AFP as well as in cells which are not active in AFP synthesis, the majority of the CCGG sites of the AFP gene are extensively methylated. In addition, we describe the existence of polymorphism in the AFP and albumin genes of Sprague-Dawley rats.

*Present address: Frederick Cancer Research Facility, National Cancer Institute, Frederick, MD, USA

+Present address: Division of Cancer Genetics, Dana-Farber Cancer Institute, Boston, MA, USA


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