Nucleic Acids Research, 1985, Vol. 13, No. 22 8165-8180
© 1985
Articles |
Growth-dependent regulation of rRNA synthesis is mediated by a transcription initiation factor (TTF-IA)
Institut für Biochemie der Universität Würzburg Röntgenring 11, D-8700 Würzburg, FRG
*To whom reprint requests should be sent
Received September 19, 1985. Revised October 28, 1985. Accepted October 28, 1985.
Mouse RNA polymerase I requires at least two chromatographically distinct transcription factors (designated TIF-IA and TIF-IB) to initiate transcription accurately and efficiently in vitro. In this paper we describe the partial purification of TIF-IA by a four-step fractionation procedure. The amount or activity of TIF-IA fluctuates in response to the physiological state of the cells. Extracts from quiescent cells are incapable of specific transcription and do not contain detectable levels of TIF-IA. Transcriptionally inactive extracts can be restored by the addition of TIF-IA preparations that have been highly purified from exponentially growing cells. During the fractionation procedure TIF-IA co-purifies with RNA polymerase I, suggesting that it is functionally associated with the transcribing enzyme. We suggest that only those enzyme molecules that are associated with TIF-IA are capable to interact with TIF-IB and to initiate transcription.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  A. Bric, C. A. Radebaugh, and M. R. Paule Photocross-linking of the RNA Polymerase I Preinitiation and Immediate Postinitiation Complexes: IMPLICATIONS FOR PROMOTER RECRUITMENT J. Biol. Chem., July 23, 2004; 279(30): 31259 - 31267. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mayer, J. Zhao, X. Yuan, and I. Grummt mTOR-dependent activation of the transcription factor TIF-IA links rRNA synthesis to nutrient availability Genes & Dev., February 15, 2004; 18(4): 423 - 434. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Claypool, S. L. French, K. Johzuka, K. Eliason, L. Vu, J. A. Dodd, A. L. Beyer, and M. Nomura Tor Pathway Regulates Rrn3p-dependent Recruitment of Yeast RNA Polymerase I to the Promoter but Does Not Participate in Alteration of the Number of Active Genes Mol. Biol. Cell, February 1, 2004; 15(2): 946 - 956. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Grummt Life on a planet of its own: regulation of RNA polymerase I transcription in the nucleolus Genes & Dev., July 15, 2003; 17(14): 1691 - 1702. [Full Text] [PDF]
|
|
|
|
|
|
A. H. Cavanaugh, I. Hirschler-Laszkiewicz, Q. Hu, M. Dundr, T. Smink, T. Misteli, and L. I. Rothblum Rrn3 Phosphorylation Is a Regulatory Checkpoint for Ribosome Biogenesis J. Biol. Chem., July 19, 2002; 277(30): 27423 - 27432. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Paule and R. J. White SURVEY AND SUMMARY Transcription by RNA polymerases I and III Nucleic Acids Res., March 15, 2000; 28(6): 1283 - 1298. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Yamamoto, A. Koga, M. Yamamoto, Y.-i. Nishi, T.-a. Tamura, Y. Nogi, and M. Muramatsu Identification of a novel 70 kDa protein that binds to the core promoter element and is essential for ribosomal DNA transcription Nucleic Acids Res., March 1, 2000; 28(5): 1199 - 1205. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. D. Hannan, W. M. Hempel, A. Cavanaugh, T. Arino, S. I. Dimitrov, T. Moss, and L. Rothblum Affinity Purification of Mammalian RNA Polymerase I. IDENTIFICATION OF AN ASSOCIATED KINASE J. Biol. Chem., January 9, 1998; 273(2): 1257 - 1267. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Gaudino and C. S. Pikaard Cytokinin Induction of RNA Polymerase I Transcription in Arabidopsis thaliana J. Biol. Chem., March 7, 1997; 272(10): 6799 - 6804. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. M. Clarke, C. L. Peterson, A. V. Brainard, and D. L. Riggs Regulation of the RNA Polymerase I and III Transcription Systems in Response to Growth Conditions J. Biol. Chem., September 6, 1996; 271(36): 22189 - 22195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. L. Riggs, C. L. Peterson, J. Q. Wickham, L. M. Miller, E. M. Clarke, J. A. Crowell, and J.-C. Sergere Characterization of the Components of Reconstituted Saccharomyces cerevisiae RNA Polymerase I Transcription Complexes J. Biol. Chem., March 17, 1995; 270(11): 6205 - 6210. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Glibetic, L. Taylor, D. Larson, R. Hannan, B. Sells, and L. Rothblum The RNA Polymerase I Transcription Factor UBF Is the Product of a Primary Response Gene J. Biol. Chem., March 3, 1995; 270(9): 4209 - 4212. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Zomerdijk, H Beckmann, L Comai, and R Tjian Assembly of transcriptionally active RNA polymerase I initiation factor SL1 from recombinant subunits Science, December 23, 1994; 266(5193): 2015 - 2018. [Abstract] [PDF]
|
|