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Nucleic Acids Research, 1985, Vol. 13, No. 23 8507-8518
© 1985

Articles

The nucleotide sequence of tobacco rattle virus RNA-2 (CAM strain)

Sandra T. Bergh, Michael G. Koziel1, Shu-Ching Huang2, Robert A. Thomas, David P. Gilley and Albert Siegel*

Department of Biological Sciences, Wayne State University Detroit, MI 48202, USA

*To whom correspondence should be addressed

Received October 21, 1985. Accepted October 30, 1985.

The nucleotide sequence of the smaller genomic strand (RNA-2) of the bipartite tobacco rattle virus (CAM strain) has been determined. RNA-2 is capped at the 5' terminus and contains 1799 nucleotide residues. There is a single 223 codon long open reading frame extending from nucleotide 574 to 1242 which designates a protein of Mr 23,654. The derived amino acid composition, in percent, matches that previously determined for the virus capsid protein. The long open reading frame is flanked by 5' and 3' untranslated regions of 573 and 554 nucleotides, respectively. The 5' leader sequence contains two different sets of direct repeats, one of 119 nucleotides and the other of 76. It also contains 13 apparently unused AUG codons, four of which lie in the same frame as the capsid protein cistron. The 3' terminal sequence of RNA-2 is identical to that of the larger genomic strand (RNA-1) for 459 nucleotides.

1Present address: Rohm and Hass Co., Spring House, PA 19477

2Present address: Department of Pharmacology, Yale University, New Haven, CT 06510, USA


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