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Nucleic Acids Research, 1985, Vol. 13, No. 23 8573-8586
© 1985

Articles

SV40 vector with early gene replacement efficient in transducing exogenous DNA into mammalian cells

Masahide Asano, Yoichiro Iwakura and Yoshimi Kawade

Institute for Virus Research, Kyoto University Sakyo-ku, Kyoto 606, Japan

Received August 29, 1985. Revised November 5, 1985. Accepted November 5, 1985.

An early replacement SV40 vector, SV40-Muß, was constructed by replacing part of the early genes of the virus with mouse interferon-ß (IFN-ß) cDNA. Upon transfection of COS-7 cells with this DNA, transducing viral particles were produced, which could infect various cells and cause efficient production of mouse IFN-ß. The viral stock contained no detectable wild-type SV40. The IFN production after the virus infection was very high in monkey kidney cells, but less so in human epithelial cells, and low in mouse, pig, hamster cells and in human lymphocytes. The efficiency of introduction of the DNA to monkey kidney cells was compared with that by the calcium phosphate precipitation method, and the viral vector was found to be more efficient by a factor of several tens to hundreds.


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 J. Biol. Chem.Home page
D. S. Strayer and D. S. Strayer
SV40 As an Effective Gene Transfer Vector in Vivo
J. Biol. Chem., October 4, 1996; 271(40): 24741 - 24746.
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