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Nucleic Acids Research, 1985, Vol. 13, No. 24 8901-8912
© 1985

Articles

Evidence for transient requirement of the IgH enhancer

Sigrid Klein*, Thomas Gerster1, Didier Picard1, Andreas Radbruch and Walter Schaffner1

Institut für Genetik der Univeisität zu Köln Weyertal 121, D-5000 Köln 41, FRG 1Institut für Molekularbiologie II der Universität Zürich Hönggerberg, CH-8093 Zürich, Switzerland

*To whom reprint requests should be sent

Received October 21, 1985. Accepted November 19, 1985.

A transcriptional enhancer is thought to play a major role in determining tissue-specific expression of the immaunoglobulin heavy chain (IgH) gene (1–3). However in three B-lymaphoid cell lines the Ig enhancer has been lost due to a spontaneous deletion, yet heavy chain synthesis persists at a high level (4–6). In the case of the enhancerless delta chain gene (5) we wanted to test whether the IgH enhancer is no longer necessary for maintenance of transcription or whether perhaps a new enhancer was created de novo by the deletion process. To this end we have cloned the relevant portion of the variant IgH gene and transfected it into myeloma and hybridoma cells. We find that the reintroduced gene segment is not expressed unless it is linked again to an enhancer. The results suggest that the IgH enhancer is necessary for the onset of transcription, presumably by organizing the gene into stable transcription complexes, but is dispensable at later stages once the transcription unit is activated.


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