Skip Navigation

This Article
Right arrow Print PDF (1378K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (22)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Labhart, P.
Right arrow Articles by Reeder, R. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Labhart, P.
Right arrow Articles by Reeder, R. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 24 8999-9009
© 1985

Articles

Xenopus ribosomal gene enhancers function when inserted inside the gene they enhance

Paul Labhart and Ronald H. Reeder

Fred Hutchinson Cancer Research Center 1124 Columbia Street, Seattle, WA 98104, USA

Received August 6, 1985. Revised October 21, 1985. Accepted November 19, 1985.

The ribosomal DNA of Xenopus laevis contains repeated sequence elements in the intergenic spacer region that enhance transcription from the adjacent gene promoter (1,2). Previous work has shown that these RNA polymerase I enhancers influence the target promoter when they are in either orientation, at a distance of several kilobases, and only when they are in cis (3–5). In this work, we further show that enhancer activity is unaffected by inserting the enhancers within the transcription unit whose promoter is being enhanced. In addition, enhancer activity does not interfere with transcription through its sequences. The results suggest that the enhancers act at a point prior to the initiation of transcription and that they are likely to be dispensable once transcription has begun.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
E. B. Mougey, L. K. Pape, and B. Sollner-Webb
Virtually the Entire Xenopus laevis rDNA Multikilobase Intergenic Spacer Serves to Stimulate Polymerase I Transcription
J. Biol. Chem., October 25, 1996; 271(43): 27138 - 27145.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
M Dunaway
A transcription factor, TFIS, interacts with both the promoter and enhancer of the Xenopus rRNA genes.
Genes & Dev., November 1, 1989; 3(11): 1768 - 1778.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.