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Nucleic Acids Research, 1985, Vol. 13, No. 4 1209-1221
© 1985

Articles

Transcriptional analysis of the adenovirus-5 EIII promoter: absence of sequence specificity for stimulation by EIa gene products

Todd Leff, Jeff Corden1, René Elkaim2 and Paolo Sassone-Corsi*

Laboratoire de Génétique Moléculaire des Eucaryotes du CNRS, Unité 184 de Biologie Moléculaire et de Génie Génétique de 1 'INSERM, Institut de Chimie Biologique, Faculté de Médecine 11, rue Humann, 67085 Strasbourg Cédex, France

*To whom reprint requests should be sent

Received November 28, 1984. Revised January 22, 1985. Accepted January 22, 1985.

To identify the adenovirus-5 EIII promoter sequences that are involved in basal level of transcription, a series of promoter deletion mutants were analyzed in vivo by transfection into HeLa cells and in vitro using a HeLa whole cell extract system. Three regions within the EIII promoter were shown to be important for efficient transcription : the TATA sequence, an upstream element centered at –55/–57, and an additional element located between –111 and –233. In vivo transcriptional analysis of EIII promoter deletions in the presence of adenovirus EIa gene products have demonstrated that the same three regions are required for EIa- stimulated transcription. We conclude that there is no sequence element in the EIII promoter between –15 and –233 that is uniquely required for the stimulation of EIII transcription by EIa gene products.

1Present address: Dept of Molecular Biology, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA

2Present address: Transgene S.A., 11, rue de Molsheim, 67000 Strasbourg, France


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