Skip Navigation

This Article
Right arrow Print PDF (5664K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Ferenz, C. R.
Right arrow Articles by Nelson, D. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ferenz, C. R.
Right arrow Articles by Nelson, D. A.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 6 1977-1995
© 1985

Articles

N-Butyrate incubation of immature chicken erythrocytes preferentially enhances the solubility of ß^ chromatin

Catherine R. Ferenz and Daniel A. Nelson

Department of Biochemical and Biophysical Sciences, University of Houston University Park, Houston, TX 77004, USA

Received November 28, 1984. Revised February 15, 1985. Accepted February 15, 1985.

The solubility of adult ß-globin chromatin (ßA chromatin) from immature chicken red blood cells can be controlled by the presence or absence of n-butyrate in a cell incuoation madium. In the absence of n-butyrate, only a small percentage (~4%) of the total ßA chromatin is in a soluble chromatin fraction following micrococcal nuclease digestion and centrifugation. This percentage increases to approxirmately 40–45% of the ßA chromatin if cells are incubated 1 hour in the presence of 10 mM sodium n-butyrate. The highest yield and enricnhent of solubilized ßA chromatin is attained when 1–4% of the DNA is rendered acid soluble, and in buffers containing 1.5 – 5 mM MgCl2 The soluble ßA nucleohistone is nucleosome oligomer size (contains DNA 250–600 bases in length) and can be separated from soluble, transcriptionally inert mononucleosomes by agarose A-5m exclusion chromatography.

The enhanced solubility appears to be specific for transcriptionally active chromatin. Whereas 40–45% of the ßA chrormatin is recovered in the supernatant fraction from n-butyrate incubated immature erythrocytes, nucleohistone containing ovalbumin DNA sequences remains insoluble.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
J. S. Smith, C. B. Brachmann, I. Celic, M. A. Kenna, S. Muhammad, V. J. Starai, J. L. Avalos, J. C. Escalante-Semerena, C. Grubmeyer, C. Wolberger, et al.
A phylogenetically conserved NAD+-dependent protein deacetylase activity in the Sir2 protein family
PNAS, June 6, 2000; 97(12): 6658 - 6663.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
V. A. Spencer and J. R. Davie
Dynamically Acetylated Histone Association with Transcriptionally Active and Competent Genes in the Avian Adult beta -Globin Gene Domain
J. Biol. Chem., September 7, 2001; 276(37): 34810 - 34815.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.