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Nucleic Acids Research, 1985, Vol. 13, No. 6 2035-2043
© 1985

Articles

Nucleotide sequence of a functional cDNA for human thymidylate synthase

Keiichi Takeishi, Sumiko Kaneda, Dai Ayusawa, Kimiko Shimizu, Osamu Gotoh* and Takeshi Seno

Department of Immunology and Virology Ina-machi, Saitama-ken 362, Japan *Department of Biochemistry, Saitama Cancer Center Research Institute Ina-machi, Saitama-ken 362, Japan

Received February 7, 1985. Accepted March 1, 1985.

We have determined the nucleotide sequence of a cDNA clone, pcHTS-1, encoding human thymidylate synthase (5,10-methylenetetrahydrofolate: dUMP C-methyltransferase, EC 2.1.1.45 [EC] ) which was previously isolated from a human fibroblast expressible cDNA library and functional in mouse cells. The 1.6 kilobase cDNA insert of pcHTS-1 encodes a subunit protein of 313 amino acid (Mr=35,706) and its predicted amino acid sequence is highly conserved in many regions including folylpolyglutamate and 5-fluoro-2'-deoxyuridylate binding sites, when compared with those of Lactobacillus casei, Escherichia coli, and bacteriophage T4. The cDNA contains in its 5'-untranslated region a triple tandemly repeated sequence consisting of 90 nucleotides, which starts immediately upstream of the ATG initiator codon, is very high in G+C content (80%), and can form three possible interconvertible stem-loop structures.


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