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Nucleic Acids Research, 1985, Vol. 13, No. 7 2583-2601
© 1985

Articles

Novel non-suppressing mutants of Escherichia coli tRNATyr su3+

Paul Furdon and Sidney Altman

Department of Biology, Yale University New Haven, CT 06520, USA

Received November 28, 1984. Revised February 27, 1985. Accepted February 27, 1985.

Several addition and deletion mutations were constructed in the region of the gene for Escherichia coli tRNATyr Formula corresponding to the dihydrouracil loop of the mature tRNA. None of these resulting mutants had detectable suppressor function compared to the parent gene yet some directed the synthesis of mature tRNA. These latter mutants may affect the ability of the tRNA to be aminoacylated or to interact with the translational machinery on the ribosome.


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