Nucleic Acids Research, 1985, Vol. 13, No. 7 2617-2629
© 1985
Articles |
Association of the lupus antigen La with a subset of U6 snRNA molecules
Max-Planck-Institut fur Molekulare Genetik, Otto-Warburg-Laboratorium D-1000 Berlin 33, FRG *Department of Molecular Biophysics and Biochemistry, Yale University New Haven, CT 06510, USA
+To whom correspondence should be sent
Received November 8, 1984. Revised February 26, 1985. Accepted February 26, 1985.
U6 snRNA is a component of the major class of small RNA-protein complexes, the Sm snRNPs, present in mammalian cell nuclei. Here we report that a substantial fraction (about 10%) of U6 RNA from human and mouse cells is associated with another lupus antigen, the 50 kd La protein. The La-bound U6 subpopulation is characterized by 3' end heterogeneity and partial undermethylation. These U6 molecules have U-rich 3' termini that could be responsible for their selective association with the La protein. The question of whether they are precursors to the major U6 RNAs found in Sm snRNPs is discussed.
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