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Nucleic Acids Research, 1985, Vol. 13, No. 8 2931-2941
© 1985

Articles

Cloning and sequence analysis of an Ig {lambda} light chain mRNA expressed in the Burkitt's lymphoma cell line EB4

M.L.M. Anderson, L. Brown, E. McKenzie, J.E. Kellow and B.D. Young*

Beatson Institute for Cancer Research, Garscube Estate Switchback Road, Bearsden, Glasgow, UK

Received February 18, 1985. Accepted March 22, 1985.

A cDNA library was constructed from the mRNA of the Ig{lambda} producing Burkitt's lymphoma cell line, EB4. Overlapping clones encompassing the coding sequence of the Ig{lambda} mRNA were isolated and sequenced. The predicted amino acid sequence shows a short hydrophobic leader peptide and a mature polypeptide of 217 residues in which V, J and C regions can be distinguished. The V region belongs to subgroup VI and has greatest homology (80%) with the Amyloid-AR protein. The constant region is the Kern– Oz+ isotype. Probing normal human DNA with the subcloned V{lambda} coding sequence detects one gene at high stringency and a family of 11 members at low stringency. To date, no restriction enzyme site polymorphisms have been detected. The V{lambda}VI gene is rearranged on both chromosomes of EB4 and is deleted on both chromosomes in the Burkitt's lymphoma cell line BL2.

*Present address: Imperial Cancer Research Fund, Lincoln's Inn Fields, London


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