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Nucleic Acids Research, 1985, Vol. 13, No. 9 3063-3082
© 1985

Articles

Molecular cloning and characterization of a gene coding for methanol oxidase in Hansenula polymorpha

A.M. Ledeboer, L. Edens, J. Maat, C. Visser, J.W. Bos, C.T. Verrips, Z. Janowicz, M. Eckart, R. Roggenkamp and C.P. Hollenberg

Unilever Research Laboratoriurn Vlaardingen P.O. Box 114, 3130 AC Vlaardingen, The Netherlands Institut für Mikrobiologie, University of Düsseldorf 4000-Düsseldorf, FRG

Received March 4, 1985. Accepted April 10, 1985.

The structural gene and the regulatory DNA sequence of the yeast Hansenula polymorpha methanol oxidase have been isolated. According to the nucleotide sequence data obtained, the structural gene encodes a 664 amino acids long protein, contains no intervening sequences, and the 5'- and 3'-non-coding region contains several sequences implicated in transcription initiation and termination in the yeast Saccharonyces cerevisiae Although the methanol oxidase is tranelocated to the peroxisomes, no cleavable signal sequence was found at the N-terminus of the protein.


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