Skip Navigation

This Article
Right arrow Print PDF (2087K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (98)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Smeekens, S.
Right arrow Articles by Weisbeck, P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Smeekens, S.
Right arrow Articles by Weisbeck, P.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 9 3179-3194
© 1985

Articles

The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone

Sjef Smeekens1, Jan van Binsbergen1 and Peter Weisbeck1,2

1Department of Molecular Cell Biology, University of Utrecht Padualaan 8, 3584 CH Utrecht, The Netherlands 2Department of Molecular Biology, University of Utrecht Padualaan 8, 3584 CH Utrecht, The Netherlands

Received February 15, 1985. Accepted April 10, 1985.

A cDNA clone (pFD1) derived from Silene pratensis ferredoxin mRNA was selected from a cDNA-library using the hybrid released translation technique. Nucleotide sequence analysis showed the cDNA insert to contain the complete coding region of the ferredoxin precursor protein. The ferredoxin precursor has a mol.wt. of 15 300, the transit-peptide has a mol.wt. of 5600. The length of the ferredoxin mRNA was found to be 700 nucleotides whereas the cDNA insert was about 1200 basepairs. S1 nuclease protection experiments showed the ferredoxin-specific DNA to be 660 basepairs in length and to start 39 nucleotides upstream of the ferredoxin coding sequ"ence.

Southern blot analysis of genomic DNA revealed the presence of only one fragment with homology to the ferredoxin cDNA probe, so it is probably a single-copy gene. Comparison of the ferredoxin transit-sequence with transit sequences of another stromal protein, the small subunit of ribulosebisphosphate carboxylase showed no apparent homology, except for a stretch of three amino acids near the processing site.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Richter and G. K. Lamppa
Structural Properties of the Chloroplast Stromal Processing Peptidase Required for Its Function in Transit Peptide Removal
J. Biol. Chem., October 10, 2003; 278(41): 39497 - 39502.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
S. Richter and G. K. Lamppa
Determinants for Removal and Degradation of Transit Peptides of Chloroplast Precursor Proteins
J. Biol. Chem., November 8, 2002; 277(46): 43888 - 43894.
[Abstract] [Full Text] [PDF]

Home page
 JCBHome page
S. Richter and G. K. Lamppa
Stromal Processing Peptidase Binds Transit Peptides and Initiates Their ATP-dependent Turnover in Chloroplasts
J. Cell Biol., October 4, 1999; 147(1): 33 - 44.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
W. Albert Rensink, M. Pilon, and P. Weisbeek
Domains of a Transit Sequence Required for in Vivo Import in Arabidopsis Chloroplasts
Plant Physiology, October 1, 1998; 118(2): 691 - 699.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. Richter and G. K. Lamppa
A chloroplast processing enzyme functions as the general stromal processing peptidase
PNAS, June 23, 1998; 95(13): 7463 - 7468.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. van 't Hof and B. de Kruijff
Characterization of the Import Process of a Transit Peptide into Chloroplasts
J. Biol. Chem., September 22, 1995; 270(38): 22368 - 22373.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
D. Schnell, F Kessler, and G Blobel
Isolation of components of the chloroplast protein import machinery
Science, November 11, 1994; 266(5187): 1007 - 1012.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.