Skip Navigation

This Article
Right arrow Print PDF (1369K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Kuchino, Y.
Right arrow Articles by Nishimura, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kuchino, Y.
Right arrow Articles by Nishimura, S.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1985, Vol. 13, No. 9 3213-3220
© 1985

Articles

Structure and transcription of the tRNA1Pro gene from Escherichia coli

Yoshiyuki Kuchino, Fumiko Mori and Susumu Nishimura

Biology Division, Nationa Cancer Center Research Institute 5-1-1, Tsukiji, Chuo-ku, Tokyo 104, Japan

Received February 5, 1985. Revised April 10, 1985. Accepted April 10, 1985.

A 5 kbp DNA fragment containing the Formula gene from Escherichia coli was cloned into Charon 21A phage and sequenced by the M13 DNA sequencing technique. When the cloned DNA fragment was used as a template for in vitro transcription with E. coli RNA polymersse, a Formula precursor of 120 nucleotide residues was obtained. The Formula gene transcribed as a single transcription unit was followed by two unusual repeating sequences, both of 108 bp. These two repeating sequences were separated by a 60 bp spacer sequence. The 5'-portion of each repeating sequence overlapped 19 bp of the 3'-terminal region of the Formula gene just like the repeating sequences in the E. coli Formula gene. A rho-independent termination was present in the first repeating unit.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
M. O'Connor
Imbalance of tRNAPro isoacceptors induces +1 frameshifting at near-cognate codons
Nucleic Acids Res., February 1, 2002; 30(3): 759 - 765.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
M. K. B. Berlyn
Linkage Map of Escherichia coli K-12, Edition 10: The Traditional Map
Microbiol. Mol. Biol. Rev., September 1, 1998; 62(3): 814 - 984.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.