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Nucleic Acids Research, 1985, Vol. 13, No. 9 3371-3388
© 1985

Articles

Effect of NusA protein on expression of the nusA, infB operon in E.coli

J.A. Plumbridge*, J. Dondon, Y. Nakamura1 and M. Grunberg-Manago

Institut de Biologie Physico-Chimique 13 rue Pierre et Marie Curie, 75005 Paris, France 1Institute of Medical Science, University of Tokyo P.O. Takanawa, Tokyo 108, Japan

Received January 2, 1985. Revised April 8, 1985. Accepted April 9, 1985.

Protein and operon fusions between lacZ and various genes of the nusA.infB operon have been constructed on {lambda} bacteriophages and used to show that the operon is negatively regulated by the level of NusA protein. Overproducing NuSA (but not IF2) from a multicopy plasmid reduces the level of ß-galactosidase from the fusions indicating repression of the operon. Introducing the {lambda} carrying the fusions into nusA mutant strains produces a higher level of ß-galactosidase-indicative of derepression of the operon. In particular. a larger form of the NusA protein which does not affect bacterial growth per se causes a derepression of the operon. As both protein and operon fusions respond equivalently, we conclude that the nusA protein is acting at the transcriptional level to regulate expression of the nusA. infB operon.

*Present address: Department of Molecular Biophysics and Biochemistry, Yale Universily, P.O. Box 6666, 260 Whitney Ave., New Haven, CT 06511, USA


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