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Nucleic Acids Research, 1986, Vol. 14, No. 13 5145-5158
© 1986

Articles

Regular distribution of length heterogeneities within non-transcribed spacer regions of cloned and genomic rDNA of Saccharomyces cerevisiae

Rune Jemtland, Eli Mæhlum, Odd S. Gabrielsen and Tordis B. Øyen

Department of Biochemistry, University of Oslo Blindern, Oslo, Norway

Received May 30, 1986. Accepted June 6, 1986.

A length difference of about 50 bp in the EcoRI fragment B of the rDNA from two different strains of Sacoharomyces cerevisiae has been mapped in detail by sequencing of cloned fragments. This 2.4 kb EcoRI fragment contains the start of the 35S rRNA gene at one end and the 5S rRNA gene in the middle flanked by non-transcribed spacers.NTS1 and NTS2. The difference appeared as short deletions or insertions in five regularly spaced regions within the 1kb NTS1, 3' to the 5S rRNA gene. The saus regions of heterogeneities were displayed when all available sequence data of the NTS1 were compared. Four of the variable regions are located 160–170 bp apart, indicating that they might represent linker sequences between phased nucleosomes. Two varient clones, differing in the length of one subfragment of NTS1. were isolated for each strain. In both cases these represented the major variants among chromosomal NTS1 as revealed by sequencing of genomic fragments.


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