Skip Navigation

This Article
Right arrow Print PDF (1007K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Cornelissen, A. W.C.A.
Right arrow Articles by Piet, B.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Cornelissen, A. W.C.A.
Right arrow Articles by Piet, B.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1986, Vol. 14, No. 14 5605-5614
© 1986

Articles

The common 5' terminal sequence on trypanosome mRNAs: a target for anti-messenger oligodeoxynucleotides

Albert W.C.A. Cornelissen, M.Philippe Verspieren1, Jean-Jacques Toulmé1, Bart W. Swinkels and Borst Piet

Division of Molecular Biology, The Netherlands Cancer Institute (Antoni van Leeuwenhoek-Huis) Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands 1Laboratoire de Biophysique, INSERM U201, CNRS ERA 481, Muséeum Nationale d'Histoire Naturelle 61 Rue Buffon, F 75005 Paris, France

Received May 30, 1986. Accepted June 26, 1986.

Several mature mRNAs of Trypanosoma brucel were previously shown to have a common 5' terminal sequence of 35 nucleotldes (nt) encoded by a separate mini-exon. To verify whether all trypanosome mRNAs contain this mini-exon sequence at their 5' end, we have tested oligodeoxynucleotides complementary to different parts of the 35 nt leader sequence for their ability to inhibit translation of total trypanosome mRNA. All ollgomers tested inhibited translation of trypanosome mRNAs in a wheat germ extract. They had no effect on translation of Brome mosaic virus mRNA and of a trypanosome mRNA for phos-phoglycerate kinase modified to remove the mini-exon sequence. Three different 12mers inhibited translation 35–60%; both the 22- and 34mer inhibited translation 95–100%. Incorporation of amino acids decreased proportionally in all protein bands detected in high resolution polyacrylamide gels. Our results show that all trypanosome mRNAs that yield a product detectable in gel contain a mini-exon sequence. He infer that most, if not all, trypanosome mRNAs contain a 5' terminal mini-exon sequence ac' quired by discontinuous synthesis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. Compagno, J. N. Lampe, C. Bourget, I. V. Kutyavin, L. Yurchenko, E. A. Lukhtanov, V. V. Gorn, H. B. Gamper Jr., and J.-J. Toulme
Antisense Oligonucleotides Containing Modified Bases Inhibit in Vitro Translation of Leishmania amazonensis mRNAs by Invading the Mini-exon Hairpin
J. Biol. Chem., March 19, 1999; 274(12): 8191 - 8198.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
E. Pascolo and J.-J. Toulme
Double Hairpin Complexes Allow Accommodation of All Four Base Pairs in Triple Helices Containing Both DNA and RNA Strands
J. Biol. Chem., September 27, 1996; 271(39): 24187 - 24192.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
C. Fulton, E. Y. Lai, and S. P. Remillard
A Flagellar Calmodulin Gene of Naegleria, Coexpressed during Differentiation with Flagellar Tubulin Genes, Shares DNA, RNA, and Encoded Protein Sequence Elements
J. Biol. Chem., March 17, 1995; 270(11): 5839 - 5848.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
V Bellofatto and G. Cross
Expression of a bacterial gene in a trypanosomatid protozoan
Science, June 9, 1989; 244(4909): 1167 - 1169.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.