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Nucleic Acids Research, 1987, Vol. 15, No. 1 31-49
© 1987

Articles

Organization and partial sequence of a DNA region of the Rhizobium leguminosarum symbiotic plasmid pRL6JI containing the genes fixABC, nifA, nifB and a novel open reading frame

Petra Grönger, Sundaram S. Manian1, Helmut Reiländer, Michael O'Connell2, Ursula B. Priefer* and Alfred Pühler

Lehrstuhl für Genetik, Fakultät für Biologie, Universität Bielefeld 4800 Bielefeld 1, FRG

*To whom reprint requests should be sent

Received October 21, 1986. Accepted December 8, 1986.

By hybridization and heteroduplex studies the fixABC and nifA genes of the Rhizobium leguminosarum symbiotic plasmid pRL6JI have been identified. DNA sequencing of the region containing nifA showed an open reading frame of 1557 bp encoding a protein of 56,178 D. Based on sequence homology, this ORF was confirmed to correspond to the nifA gene. Comparison of three nifA proteins (Klebsiella pneumoniaer Rhizobium meliloti, Rhizobium leguminosarum) revealed only a weak relationship in their N-terminal regions, whereas the C-terminal parts exhibited strong homology. Sequence analysis also showed that the R. leguminosarum nifA gene is followed by nifB and preceded by fixC with an open reading frame inserted in between. This novel ORF of 294 bp was found to be highly conserved also in R. meliloti. No known promoter and termination signals could be defined on the sequenced R. leguminosarum fragment.

1Present addresses: Department of Dairy and Food Microbiology, University College, Cork, Ireland

2Present addresses: National Institute for Higher Education, Dublin, Ireland


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