Nucleic Acids Research, 1987, Vol. 15, No. 20 8149-8166
© 1987
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A cell type specific factor recognizes the rat thyroglobulin promoter
1Centro di Endocrinologia ed Oncologia sperimentale del CNR, c/o II Facoltà di Medicina 80131 Napoli, Italy 2Laboratory of Biochemistry of the National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA
*To whom correspondence should be addressed at: European Molecular Biology Laboratory, Postfach 10.2209, 6900 Heidelberg, FRG
Received August 20, 1987. Accepted September 25, 2987.
We have fused a 900 base pair long DNA segment containing the transcriptional start site of the rat thyroglobulin (Tg) gene to the bacterial gene for chloramphenicol acetyltransferase (cat). The fusion gene has been introduced into three different cell lines derived from the rat thyroid gland and into a rat liver cell line. Expression of the fusion gene was detected only in the one thyroid cell line that is able to express the endogenous Tg gene. The minimum DNA sequence required for the cell type specific expression was determined by deletion analysis; it extends 170 nucleotides upstream of the transcription initiation site. The Tg promoter contains a readily detectable binding sites for a factor present in salt extracts of thyroid cell nuclei. This binding site is not recognized by the nuclear extracts of any other cell type that we have tested, suggesting that it may help mediate the cell type specific expression of the Tg gene.
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