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Nucleic Acids Research, 1988, Vol. 16, No. 10 4253-4268
© 1988

Articles

Analysis of the Drosophila rDNA promoter by transient expression

David C. Hayward+ and David M. Glover*

Cancer Research Campaign, Eukaryotic Molecular Genetics Research Group, Department of Biochemistry, Imperial College of Science and Technology London, SW7 2AZ, UK

* To whom correspondence should be addressed

Received March 3, 1988. Revised April 22, 1988. Accepted April 22, 1988.

We have examined the expression of the bacterial gene chloramphenicol acetyl transferase (CAT) under the control of the Drosophila rDNA promoter following transfection into Drosophila tissue culture cells. Constructs having an entire NTS, corresponding to approximately 3640 base pairs of upstream rDNA sequence, or constructs with 306 base pairs of upstream sequence respectively, are transcribed at 5 fold or 2 fold higher levels than a construct with 43 base pairs of upstream DNA. In co-transfection experiments, the construct with the entire NTS competes for transcription 20 fold more effectively than the construct with 306 base pairs of upstream sequence. Constructs having either 72 base pairs or 60 base pairs of upstream rDNA sequences, on the other hand, are transcribed very much less efficiently than constructs with either 306 bp or with only 43 bp of upstream DNA. These sequences, which reduce levels of rDNA transcription in the absence of additional upstream DNA, lie in a region in which the rDNA promoter differs from its duplications within the NTS.

+Present address: Research School of Biological Sciences, Australian National University, Canberra, ACT, Australia


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