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Nucleic Acids Research, 1988, Vol. 16, No. 11 4915-4926
© 1988

Articles

Isolation and structural analysis of a ribosomal protein gene in D.melanogaster

F. Rafti, G. Gargiulo1, A. Manzi, C. Malva, G. Grossi2, S. Andone and F. Graziani

Istituto Internazionale di Genetica e Biofisica CNR, Via Marconi 10, Naples, Italy 1Dipartimento di Genetica, Biologia Generale e Molecolare Naples, Italy 2Dipartimento di Scienze Fisiche, Universitá di Napoli Naples, Italy

Received February 11, 1988. Accepted April 26, 1988.

By using the cDNA clone containing the sequence for the L1 ribosomal protein gene of Xenopus laevis as probe (1), we have isolated positive phages from a Drosophila melanogaster genonmic library. The Drosophila genomic fragment, which gives the hybridization signal with the Xenoous cDNA, was sequenced: a region of 369 bp is 70% homologous to the sequence of X. laevis L1 cDNA. The gene was localized in situ at position 98AB of the right arm of the third polytene chromosome. By S1 mapping and heteroduplex analysis we have found that the gene is interrupted by three introns. A Drosophi1a cDNA embryonic library was screened and three cDNA clones were Isolated (900, 1400 and 1500 nt long). By Northern analysis the cDNAs identify a 1400nt transcript present at every stage of development. By the features described, the clones we have isolated identify the Drosophila rp gene homologous to the L1 rp gene of Xenoous and could code for the L1 ribosomal protein described in D. melanogaster.


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