Nucleic Acids Research, 1988, Vol. 16, No. 11 5031-5038
© 1988
Articles |
The relative importance of Escherichia coli exonuclease III and endonuclease IV for the hydrolysis of 3'-phosphoglycolate ends in polydeoxynucleotides
Biochimie, Faculté des Sciences, Université de Li`ge Sart Tilman B6, 4000 Liège I, Belgium
Received March 28, 1988. Revised May 13, 1988. Accepted May 13, 1988.
In vitro, in the presence of Mg++, the 3'-phosphoglycolatase activity of endonuclease IV is about 4-times smaller than that of exonuclease III for the same AP endonuclease activity. It thus seems that endonuclease IV has only a minor role in the repair of strand breaks limited by 3'-phosphoglycolate ends in Escherichia coli even after the amount of enzyme has been increased by induction with O2 -generating agents.
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