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Nucleic Acids Research, 1988, Vol. 16, No. 13 5741-5754
© 1988

Articles

Structure and expression of the gene coding for the {alpha}-subunit of DNA-dependent RNA polymerase from the chioroplast genome of Zea mays

Michael Ruf and Hans Kössel

Institut für Biologie III der Universität Freiburg Schänzlestrasse 1, D-7800 Freiburg, FRG

Received May 4, 1988. Accepted May 27, 1988.

The rpoA gene coding for the {alpha}-subunit of DNA-dependent RNA polymerase located on the DNA of Zea mays chloroplasts has been characterized with respect to its position on the chioroplast genome and its nucleotide sequence. The amino acid sequence derived for a 39 Kd polypeptide shows strong homology with sequences derived from the rpoA genes of other chloroplast species and with the amino acid sequence of the {alpha}-subunit from E. coli RNA polymerase. Transcripts of the rpoA gene were identified by Northern hybridization and characterized by S1 mapping using total RNA isolated from maize chioroplasts. Antibodies raised against a synthetic C-terminal heptapeptide show cross reactivity with a 39 Kd polypeptide contained in the stroma fraction of maize chloroplasts. It is concluded that the rpoA gene is a functional gene and that therefore, at least the {alpha}-subunit of plastidic RNA polymerase, is expressed in chioroplasts.


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