Skip Navigation

This Article
Right arrow Print PDF (609K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (80)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Pyle, L. E.
Right arrow Articles by Finch, L. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pyle, L. E.
Right arrow Articles by Finch, L. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 13 6015-6022
© 1988

Articles

Pulsed-field electrophoresis indicates larger-than-expected sizes for mycoplasma genomes

Louise E. Pyle, Lynn N. Corcoran1, Benjamin G. Cocks, Andrew D. Bergemann, Jane C. Whitley and Lloyd R. Finch

Russell Grimwade School of Biochemistry, University of Melbourne Parkville, Victoria 3052, Australia 1Walter and Eliza Hall Institute Parkville, Victoria 3052, Australia

Received February 12, 1988. Revised June 1, 1988. Accepted June 1, 1988.

The sizes of large DNA fragments produced from genornes of members of the Mycoplasmataceae by digestion with restriction endonucleases having infrequent (1 to 3) cleavage sites within the genome were estimated from their mobility in contour-clamped homogeneous electric field (CHEF) agarose gel electrophoresis by comparison with yeast chromosomal DNA markers. The estimates of total genome size for 7 strains of 6 species ranged from approximately 900 kilo base pairs (kb) for Ureaplasma urealyticum 960T to 1330 kb for M. mycoides subsp. mycoides GC-1176. The values derived from this new method are considerably higher than those of approximately 500 Mdaltons or 750 kb previously reported for genome sizes in members of the Mycoplasmataceae.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Int. J. Syst. Evol. Microbiol.Home page
D. R. Brown, R. F. Whitcomb, and J. M. Bradbury
Revised minimal standards for description of new species of the class Mollicutes (division Tenericutes)
Int J Syst Evol Microbiol, November 1, 2007; 57(11): 2703 - 2719.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
J. L. Allen, A. H. Noormohammadi, and G. F. Browning
The vlhA loci of Mycoplasma synoviae are confined to a restricted region of the genome
Microbiology, March 1, 2005; 151(3): 935 - 940.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
G. E. Gasparich, R. F. Whitcomb, D. Dodge, F. E. French, J. Glass, and D. L. Williamson
The genus Spiroplasma and its non-helical descendants: phylogenetic classification, correlation with phenotype and roots of the Mycoplasma mycoides clade
Int J Syst Evol Microbiol, May 1, 2004; 54(3): 893 - 918.
[Abstract] [Full Text] [PDF]

Home page
 Int. J. Syst. Evol. Microbiol.Home page
H. Neimark, B. Hoff, and M. Ganter
Mycoplasma ovis comb. nov. (formerly Eperythrozoon ovis), an epierythrocytic agent of haemolytic anaemia in sheep and goats
Int J Syst Evol Microbiol, March 1, 2004; 54(2): 365 - 371.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
A. C. Padovan, G. Firrao, B. Schneider, and K. S. Gibb
Chromosome mapping of the sweet potato little leaf phytoplasma reveals genome heterogeneity within the phytoplasmas
Microbiology, April 1, 2000; 146(4): 893 - 902.
[Abstract] [Full Text]

Home page
 Microbiol. Mol. Biol. Rev.Home page
S. Razin, D. Yogev, and Y. Naot
Molecular Biology and Pathogenicity of Mycoplasmas
Microbiol. Mol. Biol. Rev., December 1, 1998; 62(4): 1094 - 1156.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
E. Jumas-Bilak, S. Michaux-Charachon, G. Bourg, M. Ramuz, and A. Allardet-Servent
Unconventional Genomic Organization in the Alpha Subgroup of the Proteobacteria
J. Bacteriol., May 15, 1998; 180(10): 2749 - 2755.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.