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Nucleic Acids Research, 1988, Vol. 16, No. 13 6027-6039
© 1988

Articles

A physical map of the genome of Mycoplasma mycoides subspecies mycoides Y with some functional loci

Louise E. Pyle and Lloyd R. Finch

Russell Grimwade School of Biochemistry, University of Melbourne Parkville, Victoria 3052, Australia

Received March 8, 1988. Revised June 1, 1988. Accepted June 1, 1988.

A physical map is presented for the 1200 kb genome of Mycoplasma mycoides subsp. mycoides Y, locating 32 cleavage sites for 8 restriction endonucleases. The large restriction fragments involved were separated and sized by pulsed-field agarose gel electrophoresis. Their locations on the map were determined by probing Southern blots of digests with individual fragments isolated from other digests and by correlating the products of double and triple digestions. Loci for 2 ribosomal RNA operons and 2 tRNA operons have been determined by probing with cloned genes and the broad regions of the replication origin and terminus have also been outlined by in vivo labelling studies.


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